Analysis of nanomaterials and their interaction with bacterial populations

Abstract

This bachelor thesis is focused on finding appropriate methodologies to inhibit cells by applying nanoparticles on surfaces where it is necessary to maintain a highly sterile environment. The aim of this work is to examine hypotheses concerning the impact of nanoparticles of titanium dioxide on bacterial populations E. coli. The research followed scientific articles focused on titanium dioxide photocatalysis topic. In this work, we have studied the nanocomposite (titanium dioxide/silicon dioxide) mounted on a plain slide using Dip coating method. The photoactive film and the plain slide were tested in UV light and dark environments. To evaluate the interaction with the titanium dioxide surfaces and the E. coli we used the standard ISO 27447 norm and Analysis of variance. Furthermore, the bacteria were tested for respiratory activity and Live/Dead. The experimental results confirmed that the surface of the photoactive layer needs for its functionality UVA radiation.