ACS Applied Materials & Interfaces 2020
Mapping Cell Membrane Organization and Dynamics Using Soft Nano-Imprint Lithography
T. SANSEN; D. SÁNCHEZ-FUENTES; R. RATHAR; A. COLOM-DIEGO; F. EL ALAOUI; J. VIAUD; M. MACCHIONE; S. DE ROSSI; S. MATILE; R. GAUDIN; V. BÄCKER; A. CARRETERO-GENEVRIER; L. PICAS
Membrane shape is a key feature of many cellular processes, including cell differentiation, division, migration, and trafficking. The development of nanostructured surfaces allowing for the in situ manipulation of membranes in living cells is crucial to understand these processes, but this requires complicated and limited-access technologies. Here, we investigate the self-organization of cellular membranes by using a customizable and bench top method allowing to engineer 1D SiO2 nanopillar arrays of defined sizes and shapes on high-performance glass compatible with advanced microscopies. As a result of this original combination, we provide a mapping of the morphology-induced modulation of the cell membrane mechanics, dynamics and steady-state organization of key protein complexes implicated in cellular trafficking and signal transduction.
Synthesis of silica sol-gel thin films
Gel films by dip-coating: layer gel films on high-performance coverslips made of borosilicate glass of the first hydrolytic class with precision thickness No. 1.5 (0.170 ± 0.005 mm) at room temperature were prepared with a ND-DC300 dip-coater (Nadetech Innovations) equipped with an EBC10 Miniclima Device to control the surrounding temperature and relative humidity.