Promoting laccase sensing activity for catechol detection using LBL assemblies of chitosan/ionic liquid/phthalocyanine as immobilization surfaces

1-butyl-3-methylimidazolium tetrafluoroborate, Biomedical, Chitosan, Copper phthalocyanine derivative, Dip coating, Enzyme inmobilization, Lbl films, Sensors

Bioelectrochemistry
2020
Promoting laccase sensing activity for catechol detection using LBL assemblies of chitosan/ionic liquid/phthalocyanine as immobilization surfaces
C. SALVO-COMINO; C. GARCIA-HERNÁNDEZ; C. GARCÍA-CABEZON; M.L. RODRIGUEZ-MENDEZ

1-butyl-3-methylimidazolium tetrafluoroborate | Biomedical | Chitosan | Copper phthalocyanine derivative | Dip coating | Enzyme inmobilization | Lbl films | Sensors

Highlights

• The performance of a laccase biosensor is improved by using LbL films as substrate.

• LbL films contain chitosan, an ionic liquid and a copper phthalocyanine derivative.

• The combination of their complementary functionalities improves the performance.

• Improvement is due to their electrocatalytic and immobilization properties

• Biosensors with laccase on LbL layers show a LOD of 9.98·10−9 M towards catechol.

Link to source: https://www.sciencedirect.com/science/article/abs/pii/S1567539419301495 ​

Materials and instruments

Layer by layer films were prepared using a rotary dip-coater device ND-R from Nadetech (Spain). LbL films were deposited onto different substrates including glass, ZnS, quartz, mica and ITO glass. ITO glass slides were purchased from SIGMA (surface resistivity 15–25 Ω/sq). Mica slides were muscovite Mica V2 quality (Electron Microscopy Science. USA.Cat. 71857-01-10).

 

Keywords: Layer-by-Layer; Laccase; Biosensor; Catechol; Phthalocyanine

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